Genes differentially expressed in brown adipose tissue between the control mice (rhebFL/FL) and rhebAD KO mice (fabp4-cre; rhebFL/FL). Genes differentially expressed in brown adipose tissue between the control mice (rhebFL/FL) and rhebAD KO mice (fabp4-cre; rhebFL/FL)

expression profile of brown adipose tissue (interscapular) from the control or rhebAD KO mice was analyzed by mRNA array. Overall design: Mice lacking Rheb in the adipocyte lineage were generated by mating RhebFL/FL mice with fabp4-Cre conditional knockout mice. Interscapular brown adipose tissue from 3 rhebAD KO mice or 3 control littermates was pooled, respectively, extracted for total RNA, followed by mRNA array analysis.

本研究通过mRNA芯片分析了对照组或rhebAD基因敲除小鼠的肩胛间棕色脂肪组织（interscapular brown adipose tissue）的基因表达谱。
实验设计概述：通过将RhebFL/FL小鼠与fabp4-Cre条件性基因敲除小鼠交配，构建获得脂肪细胞谱系中Rheb缺失的小鼠。分别收集3只rhebAD KO小鼠与3只同窝对照小鼠的肩胛间棕色脂肪组织，各自混合后提取总RNA，随后开展mRNA芯片分析。