Gene expression in peripheral cells: effects of Foxp3 and PDE3B

This data set is comprised of all peripheral (pooled lymph nodes and spleen) T cell subsets presented in this manuscript. These include T-N, T-25, T-FN and T-R cells; T-25, T-FN and T-R cells from mice treated with IL-2; and T-R cells transduced with empty, PDE3B-expressing or PDE3B(H801A)-expressing retroviral vectors (after transfer into recipient mice). Keywords: cell type comparison, response to growth factor, genetic modification Detailed methods are presented in the manuscript and in the attached Supplementary Information. All RNA samples were derived from cells immediately after isolation from mice. Although this data set is comprised of 2 separate experiments (cells isolated from normal mice or IL-2 treated mice and cells transduced with retroviral vectors and passaged through recipient mice), the 10 expression arrays were normalized together to allow for cross comparison.

本数据集涵盖本论文中提及的所有外周T细胞亚群，其样本取自混合的淋巴结与脾脏组织。其中包括T-N、T-25、T-FN及T-R细胞；经白细胞介素2（IL-2）处理的小鼠来源的T-25、T-FN和T-R细胞；以及经空载、表达磷酸二酯酶3B（PDE3B）或表达PDE3B(H801A)的逆转录病毒载体转导后（移植至受体小鼠体内）的T-R细胞。关键词：细胞类型比较、生长因子应答、基因修饰。详细实验方法详见本论文及附带的补充材料。所有RNA样本均取自小鼠体内分离后即刻的细胞。尽管本数据集包含两项独立实验：分别为从正常小鼠或经IL-2处理的小鼠体内分离的细胞，以及经逆转录病毒载体转导并移植至受体小鼠体内传代的细胞，但10张表达芯片已进行统一归一化处理，以支持跨实验比较。