Adult male prairie vole nucleus accumbens RNAseq dataset for "Prolonged partner separation erodes nucleus accumbens transcriptional signatures of pair bonding in male prairie voles."

This dataset contains RNA sequencing results from adult male prairie voles that were in either opposite-sex or same-sex pairs that were subsequently either separated from or remained paired with their partner for either 48 hours or 4 weeks prior to collecting nucleus accumbens tissue. The goal of this experiment was to determine the nucleus accumbens transcriptional response specific to separation from an opposite-sex partner. RNA sequencing was done on polyA enriched transcripts using Illumina single-end sequencing. Samples from 3 groups were from a Ribo-seq protocol using a virally delievered, vole optimized Translating Ribosome Affinity Purification construct (Heiman et al., 2008). These samples contain files for both the input fraction and the pulldown fraction (denoted with a _P suffix). Overall design: This experiment contains 8 groups with 5-9 biological samples per group. Samples were hand dissected adult male prairie vole nucleus accumbens brain tisame-sexue according to Heiman et al., 2014. Half of the groups were males in opposite-sex pairs (experimental groups) while the other half were males in same-sex pairs (naive, control groups). Within each pairing type, animals were cohoused for 2 weeks then they were either separated or remained paired for either 48 hours or 4 weeks prior to tissue collection to assess the transcriptional trajectory of partner loss. RNA integrity and library quality was assessed by TapeStation and only samples with a RIN above 7.3 were prepared into libraries. Library preparation was via the KAPA mRNA HyperPrep kit with polyA enrichment for 1X75 RNA sequencing using Illumina NextSeq V2 for a total read depth of ~20M reads/sample. Samples that were part of the vTRAP groups have both input and pulldown files.

本数据集包含成年雄性草原田鼠的RNA测序（RNA sequencing）结果。这些田鼠被分为异性配对或同性配对组，在采集伏隔核（nucleus accumbens）组织前，分别将其与伴侣分离或维持配对状态48小时或4周。本实验的主要目的是确定仅由异性伴侣分离所特异性诱导的伏隔核转录应答。
测序针对polyA富集转录本（polyA enriched transcripts），采用Illumina单端测序（Illumina single-end sequencing）策略。另有3组样本采用基于病毒递送、田鼠优化的翻译核糖体亲和纯化（Translating Ribosome Affinity Purification）构建体的核糖体测序（Ribo-seq）方案（Heiman等，2008）。此类样本同时包含输入组分与下拉组分（以下拉组分后缀_P标注）。
本实验总体设计如下：共包含8个组，每组含5-9个生物学重复样本。所有样本均为手工解剖的成年雄性草原田鼠伏隔核脑组织，解剖方法参照Heiman等2014年的方案。其中半数组为异性配对的雄性田鼠（实验组），剩余半数为同性配对的雄性田鼠（对照组）。在每种配对类型中，动物先群居饲养2周，随后将其与伴侣分离或维持配对状态48小时或4周，再采集组织以评估伴侣丧失后的转录组动态变化。
采用TapeStation仪器评估RNA完整性与文库质量，仅选取RNA完整性数（RNA Integrity Number, RIN）高于7.3的样本进行建库。文库构建采用KAPA mRNA HyperPrep建库试剂盒（KAPA mRNA HyperPrep kit），结合polyA富集步骤，使用Illumina NextSeq V2测序平台（Illumina NextSeq V2）进行1×75 bp的RNA测序，单样本总测序深度（read depth）约为20M条reads。属于vTRAP组的样本同时包含输入与下拉组分文件。