Genes responsive to bisphenol A in testicular Sertoli cells

We performed global scale microarray analysis to identify detailed mechanisms by which bisphenol A (BPA) induce cell death by using an Affymetrix GeneChip system. Testicular Sertoli TTE3 cells used in the present study were derived from transgenic mice harboring a temperature-sensitive simian virus 40 large T-antigen. Cell death accompanying endoplasmic reticulum stress was observed in the cells treated with 0.2 mM BPA. Of the 22,690 probe sets analyzed, approximately 1,300 genes were down- and up-regulated by a factor of 2.0 or greater in the cells treated with BPA. Keywords: bisphenol A, gene expression, testicular Sertoli cell TTE3 cells were treated with BPA for 0, 3, 6, and 12 h. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with the Mouse Expression Array 430A which was spotted with 22,690 probe sets. Sample preparation for array hybridization was carried out as described in the manufacture’s instructions.

本研究采用Affymetrix GeneChip系统开展全基因组范围的微阵列分析，旨在阐明双酚A（bisphenol A，BPA）诱导细胞死亡的具体分子机制。本研究使用的睾丸支持细胞TTE3（Testicular Sertoli TTE3），源自携带温度敏感性猴病毒40大T抗原的转基因小鼠。经0.2 mM双酚A处理的细胞中，观察到伴随内质网应激的细胞死亡现象。在本次分析的22690个探针组（probe sets）中，经双酚A处理的细胞内约有1300个基因的表达量发生了2.0倍及以上的上调或下调。
关键词：双酚A、基因表达、睾丸支持细胞
将TTE3细胞用双酚A分别处理0、3、6和12小时。从细胞中提取总RNA样本。采用搭载22690个探针组的小鼠表达芯片430A（Mouse Expression Array 430A），通过Affymetrix GeneChip®系统分析基因表达情况。芯片杂交的样本制备严格按照制造商的说明书操作完成。