Supplementary data: Description of two new subspecies of Bacillus cabrialesii.

Strain TSO2T, a plant growth-promoting rhizobacteria and biological control agent, was isolated from wheat rhizosphere sampled from the Yaqui Valley in Mexico. The strain was identified using a polyphasic approach. Based on its analysis of the full-length 16S rRNA gene, strain TSO2T was assigned to the genus<em> Bacillus</em>, which was supported by morphological and metabolic traits, such as Gram-positive staining, rod shape, spore formation, strictly aerobic metabolism, catalase-positive activity, starch, and casein hydrolysis, reduction of nitrate to nitrite, growth in presence of lysozyme and 2% NaCl, citrate utilization, growth at pH 6.0, acid production from glucose and indole production from tryptophan. Additionally, strain TSO2T possesses swarming motility, presenting a featureless mat pattern that can cover the whole petri dish. The whole-genome phylogenetic relationship analysis elucidated that strain TSO2T is closely related to <em>Bacillus cabrialesii</em> TE3T. The maximum values for average nucleotide identity (ANI) and <em>in silico</em> DNA–DNA hybridization from the genome-to-genome distance calculator (GGDC) were 97% and 73.4%, respectively, related to <em>Bacillus cabrialesii</em> TE3T, where both ANI and GGDC values were barely above the species delimitation threshold, but below the subspecies limit. Also, strain TSO2T showed the ability to produce a fatty acid (C18:0) that is not present in closely related <em>Bacillus</em> species. These results provide evidence that strain TSO2T is a novel subspecies of the species <em>Bacillus cabrialesii</em>, for which the name <em>Bacillus cabrialesii</em> subsp. <em>mobilis</em> subsp. nov. is proposed. The type strain of <em>Bacillus cabrialesii</em> subsp. <em>mobilis</em> subsp. nov. is TSO2T (CM-CNRG TB52T = LBPCV TSO2T). The description of this novel subspecies automatically creates the subspecies <em>Bacillus cabrialesii</em> subsp. <em>cabrialesii </em>subsp. nov. for which the type strain is TE3T (CM-CNRG TB54T= CCStamb A1T).

菌株TSO2^T是一株植物促生根际细菌（plant growth-promoting rhizobacteria）兼生防菌（biological control agent），分离自墨西哥亚基河谷的小麦根际土壤样本。该菌株采用多相分类法进行鉴定。基于全长16S rRNA基因序列分析，菌株TSO2^T被归为芽孢杆菌属（*Bacillus*），该分类结果得到形态与代谢特征的支持，包括革兰氏阳性染色、杆状形态、芽孢形成、严格需氧代谢、过氧化氢酶阳性活性、淀粉与酪蛋白水解、硝酸盐还原为亚硝酸盐、可在溶菌酶及2%氯化钠环境中生长、柠檬酸盐利用、pH 6.0条件下可生长、可利用葡萄糖产酸以及可利用色氨酸产生吲哚。此外，菌株TSO2^T具有群集运动能力，可形成无特异性结构的菌膜，能够覆盖整个培养皿。全基因组系统发育关系分析表明，菌株TSO2^T与卡布里亚尔芽孢杆菌（*Bacillus cabrialesii*）TE3^T亲缘关系密切。基于基因组到基因组距离计算器（GGDC, genome-to-genome distance calculator）的分析结果，其与卡布里亚尔芽孢杆菌TE3^T的平均核苷酸一致性（ANI, average nucleotide identity）和数字化DNA-DNA杂交（in silico DNA–DNA hybridization）值分别为97%和73.4%，两项指标均仅略高于物种界定阈值，但低于亚种划分阈值。此外，菌株TSO2^T可产生一种亲缘关系相近的芽孢杆菌属物种所不具备的脂肪酸C18:0。以上结果证实，菌株TSO2^T是卡布里亚尔芽孢杆菌的一个新亚种，故提议将其命名为卡布里亚尔芽孢杆菌运动新亚种（*Bacillus cabrialesii* subsp. *mobilis* subsp. nov.）。卡布里亚尔芽孢杆菌运动新亚种的模式菌株为TSO2^T（CM-CNRG TB52^T = LBPCV TSO2^T）。该新亚种的发表自动确立了卡布里亚尔芽孢杆菌卡布里亚尔新亚种（*Bacillus cabrialesii* subsp. *cabrialesii* subsp. nov.），其模式菌株为TE3^T（CM-CNRG TB54^T = CCStamb A1^T）。