Plasma protein corona on superparamagnetic iron oxide nanoparticles (SPIONs)

We determined the composition of the plasma protein corona on silica-coated versus dextran-coated iron oxide nanoparticles using mass spectrometry-based proteomics approaches. Gene ontology (GO) enrichment analysis and Ingenuity Pathway Analysis (IPA) revealed distinct protein corona compositions for the two different SPIONs. Relaxivity of silica-coated SPIONs was modulated by the presence of a protein corona. Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.

本研究采用基于质谱的蛋白质组学方法，解析了二氧化硅包被与葡聚糖包被的氧化铁纳米颗粒表面的血浆蛋白冠（protein corona）组成。通过基因本体（GO）富集分析与Ingenuity Pathway Analysis（IPA）通路分析，发现两种不同的超顺磁性氧化铁纳米颗粒（SPIONs）的蛋白冠组成存在显著差异。二氧化硅包被SPIONs的弛豫率会因蛋白冠的存在而受到调节。此外，原代人单核细胞来源的巨噬细胞的细胞活力会受到二氧化硅包被SPIONs表面蛋白冠的影响，但不受葡聚糖包被SPIONs表面蛋白冠的影响；蛋白冠可促进二氧化硅包被SPIONs的细胞摄取，但对葡聚糖包被SPIONs的细胞内化过程无显著影响。