CIL:37103, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library

Specifications of the custom built two-photon microscope, including the laser sources, signal amplification, PMT specifications, and YFP filter sets are described in:
Ruthazer ES, Li J, Cline HT. 2006. Stabilization of axon branch dynamics by synaptic maturation. J Neurosci 26(13):3594-3603.

The methods of cell transfection with electroporation can be found here:
Bestman JE, Ewald RC, Chiu S-L, Cline HT. 2006. In vivo single-cell electroporation for transfer of DNA and macromolecules. Nat Protocols 1(3):1267-1272.

Further explanation can be found here:
Bestman JE, Cline HT. 2008. The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo. Proc Natl Acad Sci U S A 105(51):20494-20499.

Bestman JE, Cline HT. 2009. The Relationship between Dendritic Branch Dynamics and CPEB-Labeled RNP Granules Captured in Vivo. Front Neural Circuits 3:10.

定制双光子显微镜的规格参数（涵盖激光光源、信号放大模块、光电倍增管（PMT）规格以及黄色荧光蛋白（YFP）滤光片组）详见下述文献：
Ruthazer ES、Li J、Cline HT. 2006. 突触成熟对轴突分支动态的稳定作用. 《Journal of Neuroscience》26(13):3594-3603.

采用电穿孔法进行细胞转染的实验方法可参阅如下文献：
Bestman JE、Ewald RC、Chiu S-L、Cline HT. 2006. 用于DNA与大分子转移的活体内单细胞电穿孔法. 《Nature Protocols》1(3):1267-1272.

更多相关说明可参见以下文献：
Bestman JE、Cline HT. 2008. RNA结合蛋白CPEB在活体内调控树突形态发生与神经元环路组装. 《美国国家科学院院刊》105(51):20494-20499.

Bestman JE、Cline HT. 2009. 活体内捕获的树突分支动态与CPEB标记的核糖核蛋白颗粒之间的关联. 《Frontiers in Neural Circuits》3:10.