Expression profiling of dexamethasone inducible expression of YFP-CPR5-C in Arabidopsis

Identification of early transcriptional responses to CPR5 function loss in Arabidopsis Expression of CPR5 C terminal half efficiently interferes with native CPR5 protein function and causes a dominant negative effect. Four-week-old leaves of Col-0 WT and transgenic Dex:YFP-CPR5-C plant were sprayed with water or 50 uM dexamethasone to induce expression of CPR5-C. Samples were collected at 24 hours after treatment. Three biological replicates were performed per genotype per treatment.

拟南芥CPR5功能丧失早期转录应答的鉴定。CPR5的C端半段的表达可有效干扰内源CPR5蛋白的功能，并引发显性负效应。选取四周龄的Col-0野生型（Col-0 WT）及转基因株系Dex:YFP-CPR5-C的叶片，分别喷施清水或50 μM地塞米松以诱导CPR5-C的表达。于处理后24小时收集样本。每种基因型、每种处理均设置三次生物学重复。