Porphyromonas gingivalis W83:Transcriptome profiling and functional verification of flavodoxin

The aim of the study was to identify the role of flavodoxin gene in the virulence of P. gingivalis W83.The mRNA profiles of P. gingivalis W83 and flavodoxin mutant strain were generated by deep sequencing, in triplicate, using Illumina sequencing platform. Differential expression analysis of two groups (Three biological replicates per group) was performed using the DESeq R package. In total, 376 genes met the DEGs criteria (194 upregulated genes and 182 down-regulated genes). The KEGG pathway analysis indicated the DEGs were significantly enriched in three KEGG pathways, comprising pathways associated with Peroxisome, Two-component system and Quorum sensing.Importantly, we found that the flavodoxin gene can regulate the type IX secretion system and oxidative stress system in P. gingivalis W83. The knowledge obtained will help us to deeply understand the role of flavodoxin gene in the P. gingivalis W83 genome. Overall design: Comparative transcriptomic analysis on the P. gingivalis W83 and the flavodoxin mutant strain

本研究旨在明确黄素氧还蛋白（flavodoxin）基因在牙龈卟啉单胞菌W83（P. gingivalis W83）毒力中的作用。本研究采用Illumina测序平台，对牙龈卟啉单胞菌W83及黄素氧还蛋白突变株进行3次生物学重复的深度测序，获取二者的mRNA转录谱。使用DESeq R包对两组样本（每组设置3次生物学重复）开展差异表达分析，最终共筛选得到376个符合差异表达基因（DEGs, differentially expressed genes）筛选标准的基因，其中上调基因194个，下调基因182个。KEGG通路分析结果显示，上述差异表达基因显著富集于3条KEGG通路，分别为过氧化物酶体（Peroxisome）通路、双组分调控系统（Two-component system）通路以及群体感应（Quorum sensing）通路。值得注意的是，本研究发现黄素氧还蛋白基因可调控牙龈卟啉单胞菌W83的IX型分泌系统与氧化应激系统。本研究所得结果有助于深入解析黄素氧还蛋白基因在牙龈卟啉单胞菌W83基因组中的功能。整体实验设计：针对牙龈卟啉单胞菌W83与黄素氧还蛋白突变株开展对比转录组学分析。