IRF4 ChIP-on-chip for Myeloma Cell Lines. Homo sapiens

To identify IRF4 transcription factor binding on chromatin at 5' regulatory regions of genes in myeloma cell line models. Keywords: binding site identification design Overall design: Formaldehyde cross-linked, sonicated chromatin is prepared from cell lines Kms12 (test) and Ly19 (control). Chromatin immunoprecipated with anti-IRF4 antibody is labeled with Cy5 and co-hybridized on Agilent Human Promoter Set arrays with chromatin immunoprecipated with normal sera labeled with Cy3 . Two biological replicates were performed for the Kms12 and Ly19 experiments.

本数据集旨在鉴定多发性骨髓瘤细胞系模型中基因5'调控区染色质上的IRF4转录因子（IRF4 transcription factor）结合情况。
关键词：结合位点鉴定设计
实验设计：从细胞系Kms12（实验组）与Ly19（对照组）中制备经甲醛交联、超声破碎的染色质；以抗IRF4抗体进行染色质免疫沉淀得到的产物用Cy5荧光标记，与以正常血清进行免疫沉淀并以Cy3荧光标记的染色质产物共同在安捷伦人类启动子组芯片（Agilent Human Promoter Set arrays）上进行杂交。针对Kms12与Ly19的实验均完成了两次生物学重复。