Supplementary Material: Defining Chaperone-Usher Fimbriae Repertoire in Serratia marcescens

Supplementary Material

Supplementary File 1. Fimbrial Usher Protein (FUP) consensus sequences for α, β, γ1, γ2, γ3, γ4, κ, and π families were built with cons tool (http://www.bioinformatics.nl/cgi-bin/emboss/cons) and used as query in a tBLASTn against S. marcescens genomes.

Supplementary File 2. Genomic coordinates of S. marcescens FUP-coding genes. Each row corresponds to a S. marcescens sequenced strain. Each CU operon family and given name is indicated on top. Nucleotide start of each FUP-coding gene in the associated genome is indicated in individual cells.

Supplementary File 3. CLUSTALX v2.0 alignment of the 421 FUP of S. marcescens along with 189 FUP from different Gram-negative bacteria. The S. marcescens isolate name along with the here assigned CU fimbriae nomenclature associated is indicated. GenBank accession numbers of non-S. marcescens FUPs along with their previously assigned family are also indicated.

Supplementary File 4. Growth curves at 37°C of S. marcescens SmUNAM836 (A) or Db10 (B) strains, carrying pSEVA246 plasmid. Data represent the mean ± SD from two independent experiments.

补充材料

补充文件1。针对α、β、γ1、γ2、γ3、γ4、κ及π家族的菌毛 usher蛋白（Fimbrial Usher Protein, FUP），通过cons工具（http://www.bioinformatics.nl/cgi-bin/emboss/cons）构建其共有序列，并将该序列作为查询序列，用于针对粘质沙雷氏菌（Serratia marcescens, S. marcescens）基因组的tBLASTn比对。

补充文件2。粘质沙雷氏菌FUP编码基因的基因组坐标信息。每一行对应一株已测序的粘质沙雷氏菌菌株；表头标注了各CU操纵子（Chaperone-Usher operon, CU operon）家族及其指定名称；各单元格内分别标注了对应基因组中每一个FUP编码基因的核苷酸起始位点。

补充文件3。采用CLUSTALX v2.0对421条粘质沙雷氏菌FUP序列与189条来自不同革兰氏阴性菌的FUP序列进行多序列比对的结果。表格中标注了各粘质沙雷氏菌分离株名称及其对应本次研究指定的CU菌毛命名规则；非粘质沙雷氏菌FUP的GenBank登录号及其此前已被分配的家族分类信息也一并列出。

补充文件4。携带pSEVA246质粒的粘质沙雷氏菌SmUNAM836（A）与Db10（B）菌株在37℃条件下的生长曲线。数据为两次独立实验所得的平均值±标准差（standard deviation, SD）。