A chromatin immunoprecipitation database for prokarytic organisms [NAP]

Bacterial transcription factors (TFs) regulate gene expression to adapt to changing environments; when combined, the TF’s regulatory actions comprise transcriptional regulatory networks (TRNs). The chromatin immunoprecipitation (ChIP) assay is the major contemporary method for mapping in vivo protein-DNA interactions in the genome. It enables the genome-wide study of transcription factor binding sites (TFBSs) and gene regulation. Although rapidly accumulating publicly-available ChIP data are a valuable resource for the study of gene regulation, there are no full datasets of key regulators and nucleoid associated proteins (NAPs) in E. coli K-12 MG1655. Here, we present the genome-wide binding for dozens of major TFs and NAPs in the E. coli K-12 MG1655. Identification of genome-wide bindings for nucleoid associated proteins in E. coli K-12 MG1655, using ChIP-exo technology

细菌转录因子（Transcription Factors, TFs）通过调控基因表达以适应环境变化，其协同调控作用共同构成转录调控网络（Transcriptional Regulatory Networks, TRNs）。染色质免疫共沉淀（Chromatin Immunoprecipitation, ChIP）是当前绘制基因组内体内蛋白质-DNA互作图谱的主流技术，可实现全基因组水平的转录因子结合位点（Transcription Factor Binding Sites, TFBSs）与基因调控研究。尽管快速积累的公开可用ChIP数据为基因调控研究提供了宝贵资源，但大肠杆菌K-12 MG1655中关键调控因子与类核结合蛋白（Nucleoid Associated Proteins, NAPs）的完整数据集仍存在空白。本研究报道了大肠杆菌K-12 MG1655中数十种主要转录因子与类核结合蛋白的全基因组结合图谱，并采用ChIP-exo技术鉴定了该菌株中类核结合蛋白的全基因组结合位点。