Hypoxic response in wild type and HIF-1alpha null hepatoctyes. Mus musculus

The primary aim of this study was to evaluate the changes in hepatocyte gene expression under short-term hypoxic conditions in wild type and HIF-1a null cultures. To this end, hypoxia treated cultures were subjected to incubation with 1% O2/5% CO2/94% N2 at 37° C for eight hours prior to RNA isolation. Duplicate normoxic controls were established from separate animals wherein cultures were untreated and treated with Adbgal. Biological triplicates of wild type and HIF-1a null cultures were placed under hypoxic conditions and subsequently processed for microarray analysis. A total of 10 microarray hybridizations were performed. Keywords: repeat sample

本研究的核心目的为评估野生型（wild type）与HIF-1α敲除（HIF-1a null）肝细胞培养物在短期低氧条件下的基因表达变化。为此，在进行RNA分离前，低氧处理组的细胞培养物需置于37℃、1%O₂/5%CO₂/94%N₂的环境中孵育8小时。本研究从不同动物中建立了两份常氧对照组培养物，其中一组未进行任何处理，另一组以Adbgal进行处理。针对野生型与HIF-1α敲除的肝细胞培养物，各设置三份生物学重复并置于低氧环境，随后进行微阵列（microarray）分析。本研究共计完成10次微阵列杂交实验。关键词：重复样本