Biochemical and photochemical mechanisms that produce different UV-induced mutation spectra

This dataset is associated with the paper entitled "<i>Biochemical and photochemical mechanisms that produce different UV-induced mutation spectra</i>", by Sugiyama, Keinard, Best, and Sanyal.<br>"Fastq.zip" contains all fastaq raw data files that have been used in the paper. Fastaq file name contains barcode # (e.g. 048.fastq) and stored in folders with NGS ID (19C, 19D etc). These identifications are used in Table S1 of the paper, which summarizes experimental conditions. <br>"NGS Protocol 4.2.docx" is our laboratory protocol for basic analysis of our NGS data. This protocol is designed for students to compute mutation frequency from fastaq data. Protocol uses four Excel template files with macro, which are also included in this dataset. The protocol and macros are designed for the specific data analysis, not for general use. Macro works only for specific template sequences used in the paper.

本数据集与Sugiyama、Keinard、Best及Sanyal合著的题为《产生不同紫外线诱导突变谱的生化与光化学机制》的论文相关联。
「Fastq.zip」压缩包内含本论文中使用的全部FASTQ（Fastq）格式原始测序数据文件。FASTQ文件的文件名包含条形码编号（例如048.fastq），并存储在以NGS（Next-Generation Sequencing，下一代测序）标识（19C、19D等）命名的文件夹中。上述标识对应论文附表S1中的内容，该附表汇总了全部实验条件。
「NGS Protocol 4.2.docx」是本实验室用于下一代测序（NGS）数据基础分析的实验规程，专为学生设计，可通过FASTQ数据计算突变频率。本规程配套4个带宏的Excel模板文件，上述文件亦包含在本数据集中。本规程与配套宏仅针对本论文的特定数据分析场景开发，不适用于通用数据分析场景，且宏仅可与论文中使用的特定模板序列配合运行。