Complete mitogenome and phylogenetic analysis of Brassica oleracea L. var. italica Plenck

In this study, we assembled and annotated the mitochondrial genome (mitogenome) of B. oleracea L. var. italica Plenck. This mitogenome was found to span 219 964 bp, with a GC content of 45.25%. It comprised 61 genes, including 35 protein-coding, 23 transfer RNA, and three ribosomal RNA genes. Among these, only 11 genes contained introns. Codon preference analysis revealed a bias toward codons ending in A/U bases. A phylogenetic analysis demonstrated a close relationship between B. oleracea L. var. italica Plenck, B. oleracea L. botrytis, and B. oleracea var. capitata. This reference mitogenome provides a basis for research on genetic conservation, phylogenetic relationships, and molecular breeding strategies among members of the Brassica genus.

本研究组装并注释了芸薹属甘蓝变种青花菜（Brassica oleracea L. var. italica Plenck）的线粒体基因组（mitogenome）。该线粒体基因组全长219964 bp，GC含量为45.25%。其共包含61个基因，其中包括35个蛋白编码基因、23个转运RNA（transfer RNA）基因以及3个核糖体RNA（ribosomal RNA）基因。在这些基因中，仅11个基因含有内含子。密码子偏好性分析结果显示，该基因组存在以A/U碱基结尾的密码子偏好现象。系统发育分析表明，青花菜、花椰菜与结球甘蓝三者间亲缘关系较近。该参考线粒体基因组可为芸薹属物种的遗传保护、系统发育关系研究以及分子育种策略研发提供基础支撑。