H3K27ac Signal Track for DU145

Genome-wide signal tracks for each sample were generated in two stages: (i) assess ChIP–seq quality and obtain the predominant fragment length using phantompeakqualtools (https://code.google.com/archive/p/phantompeakqualtools/); (ii) use align2rawsignal (https://code.google.com/archive/p/align2rawsignal/wikis/Method.wiki) to generate signal track (parameters: -n = 5, -k = epanechnikov, -l = [frag-ment length from step (i)], -w = 150, -f = 0).

所有样本的全基因组信号轨道（genome-wide signal tracks）均通过两个阶段生成：① 利用phantompeakqualtools工具评估染色质免疫共沉淀测序（ChIP-seq）质量，并获取样本的优势片段长度，工具访问地址为：https://code.google.com/archive/p/phantompeakqualtools/；② 借助align2rawsignal工具生成信号轨道，参数设置为：-n=5，-k=epanechnikov（埃帕涅奇科夫核），-l=步骤①中得到的片段长度，-w=150，-f=0，工具方法页面地址为：https://code.google.com/archive/p/align2rawsignal/wikis/Method.wiki。