Oligonucleotides used for EMSA and site-directed mutagenesis.

Fwd, forward; Rev, reverse; n, every base; PP, proximal promoter.The numbers in brackets refer to the transcription start site of UbC gene identified as +1. The mutagenized Sp1 and YY1 binding sequences are in bold letters and nucleotide changes respect to the wild-type sequence are underlined. Sp1 and YY1 consensus sequences are also indicated in the third column. The italicized lower case letters in the primers used for splice site mutagenesis indicate the upper and downstream exon flanking sequences.(1)YY1 binding motifs in the UbC intron are in the minus (antisense) strand; the one in the upstream proximal promoter (PP) sequence is in the sense strand.

Fwd（正向，forward）、Rev（反向，reverse）；n代表每碱基；PP为近端启动子（proximal promoter）。括号内的数字对应被定义为+1位的UbC基因转录起始位点。经诱变的Sp1与YY1结合序列以粗体标注，相对于野生型序列的核苷酸变异位点以下划线标出。Sp1与YY1的共有序列（consensus sequence）亦在第三列中标注。用于剪接位点诱变的引物中，斜体小写字母代表上游及下游的外显子侧翼序列。(1) UbC基因内含子中的YY1结合基序位于负义（反义，minus/antisense）链；而上游近端启动子（PP）序列中的YY1结合基序位于正义链。