T-5224, a selective inhibitor of c-Fos/AP-1, suppresses IgE-mediated mast cell activation†. T-5224, a selective inhibitor of c-Fos/AP-1, suppresses IgE-mediated mast cell activation†

Purpose:The goal of this study is to asses the gene expression changes in non-stimulated mast cell and anti-IgE-stimulated mast cell Methods:RBL-2H3 mRNA profiles of non-stimulated mast cell and anti-IgE-stimulated mast cell were generated by deep sequencing, in triplicate. Differential expression analysis was performed using the DESeq2(v1.4.5) with Q value ≦0.05, following the heatmap was drawn by pheatmap(v1.0.8) . Results:Compared with non-stimulated mast cell, anti-IgE-stimulated mast cell induced a robust transcriptional response, with 191 differentially expressed genes following anti-IgE treatment (116 upregulated and 75 downregulated). Among the up-regulated genes. Conclusions:Our study represents the first detailed analysis of RBL-2H3 transcriptomes, with biologic replicates, generated by RNA-seq technology. Overall design: RBL-2H3 mRNA profiles of non-stimulated mast cell and anti-IgE-stimulated mast cell

研究目的：本研究旨在评估未刺激肥大细胞（mast cell）与抗IgE（anti-IgE）刺激肥大细胞的基因表达变化。
实验方法：通过深度测序生成未刺激肥大细胞与抗IgE刺激肥大细胞的RBL-2H3细胞mRNA表达谱，每组样本均设置三次生物学重复。采用DESeq2(v1.4.5)进行差异表达分析，筛选阈值为q值≤0.05；后续使用pheatmap(v1.0.8)绘制差异表达基因热图。
实验结果：与未刺激肥大细胞相比，抗IgE刺激的肥大细胞可引发强烈的转录应答，经抗IgE处理后共鉴定出191个差异表达基因，其中116个基因上调、75个基因下调。在上调基因中。
研究结论：本研究首次借助RNA测序（RNA-seq）技术，结合生物学重复样本，完成了RBL-2H3细胞转录组的详细分析。
实验设计概况：未刺激肥大细胞与抗IgE刺激肥大细胞的RBL-2H3细胞mRNA表达谱