miRNA-seq of grapefruit exosomes and fusion vesicles

miRNA-sequencing of grapefruit-derived extracellular vesicles and fusion nanovesicles derived from grapefruit-derived extracellular vesicles and gingival mesenchymal stem cell-derived vesicles. We then performed gene expression profiling analysis to explore the miRNAs derived from grapefruit-derived extracellular vesicles, and the retention rate of miRNAs after membrane fusion Comparative gene expression profiling analysis of miRNA-seq data for grapefruit exosomes and fusion vesicles derived from grapefruit exosomes, each group had three biological replicates.

本研究对葡萄柚来源的细胞外囊泡（extracellular vesicles），以及由葡萄柚来源细胞外囊泡与牙龈间充质干细胞（gingival mesenchymal stem cell）来源囊泡融合形成的融合纳米囊泡（fusion nanovesicles）开展miRNA测序（miRNA-sequencing）。随后通过基因表达谱分析，探究葡萄柚来源细胞外囊泡所携带的miRNA，以及膜融合后miRNA的保留率。此外，本研究还针对葡萄柚外泌体（exosomes）及其衍生融合囊泡的miRNA测序数据，进行了对比基因表达谱分析，每组设置3个生物学重复（biological replicates）。