Differential dynamic plasma miRNA profiles during the peri-implantation period in patients with different reproductive outcomes following embryo transfer

To investigate the differential dynamic plasma miRNA profiles during the peri-implantation period in patients with different reproductive outcomes following embryo transfer. 10 negative pregnancy (NP) patients, 10 biochemical pregnancy loss (BPL) patients, and 10 clinical pregnancy (CP) patients were enrolled for miRNA-seq in this study. Peripheral blood samples were collected at three time-points (ET0, ET11, and ET14) from BPL patients and CP patients, and samples were collected at two time-points (ET0 and ET11) from NP patients. Small RNA libraries were constructed using peripheral plasma RNA. miRNA-seq was used to investigate the differential dynamic peripheral miRNA changes between the three groups during the peri-implantation period. Overall design: We prospectively recruited a cohort undergoing embryo transfer (ET). Peripheral llood samples were collected on ET day before ET (ET0), 11 days after ET (ET11), and 14 days (ET14) after ET. The patients were divided into the negative prengancy (NP), biochemical pregnancy loss (BPL), and clinical pregnancy (CP) groups according to serum hCG levels. Ten patients in each group were randomly selected for miRNA-seq.

本研究旨在探究胚胎移植（embryo transfer, ET）后不同妊娠结局患者的围植入期外周血浆微小核糖核酸（microRNA, miRNA）动态差异表达谱。本研究纳入妊娠阴性（negative pregnancy, NP）、生化妊娠丢失（biochemical pregnancy loss, BPL）及临床妊娠（clinical pregnancy, CP）患者各10例，均接受miRNA测序（miRNA-seq）。BPL与CP患者于胚胎移植当日（ET0）、移植后11天（ET11）及移植后14天（ET14）三个时间点采集外周血样本；NP患者则于ET0及ET11两个时间点采集样本。研究团队采用外周血浆RNA构建小RNA文库，通过miRNA-seq分析三组患者围植入期外周血浆miRNA的动态差异变化。整体研究设计：本研究前瞻性招募接受胚胎移植（embryo transfer, ET）的队列，于胚胎移植当日（ET0，移植前采样）、移植后11天（ET11）及移植后14天（ET14）采集外周血样本；根据血清人绒毛膜促性腺激素（hCG）水平将患者分为妊娠阴性（negative pregnancy, NP）、生化妊娠丢失（biochemical pregnancy loss, BPL）及临床妊娠（clinical pregnancy, CP）三组，每组随机选取10例患者进行miRNA测序（miRNA-seq）。