Assessment of gene expression changes during human cortical interneuron development. Assessment of gene expression changes during human cortical interneuron development

We have used our protocol for generating cortical interneurons from human embryonic stem cells (hESCs) to study gene expression changes during this process, to identify regulatory networks critical to cortical interneuron development. Samples were collected at day 0 (hESCs), day 15 (ventral telencephalic patterned medial ganglionic eminence-like progenitors), day 35 (immature interneurons), and day 60 (mature interneurons). Overall design: For each time point, 3-4 biological replicates generated from independant differentiations of hESCs were used for transcriptomic analysis by RNA-sequencing.

本研究采用我们建立的从人类胚胎干细胞（human embryonic stem cells, hESCs）诱导生成皮层中间神经元的实验方案，探究该诱导过程中的基因表达变化，以筛选出调控皮层中间神经元发育的关键网络。
样本采集于诱导第0天（人类胚胎干细胞，即hESCs）、第15天（腹侧端脑模式化的内侧神经节隆起样前体细胞）、第35天（未成熟中间神经元）以及第60天（成熟中间神经元）。
总体实验设计：针对每个时间点，均采用3~4份源自独立人类胚胎干细胞分化实验的生物学重复样本，通过RNA测序（RNA-sequencing）开展转录组学分析。