Interaction between NIH:OVCAR-3 cells and mesenchymal stroma/stem cells (MSC)

A co-culture of 60% GFP-labeled human mesenchymal stem cells (MSCGFP) and 40% mcherry-labeled NIH:OVCAR-3 tumor cells were incubated in MSC culture medium for up to 7 days in cell culture plates (diameter 10 cm, Greiner BioOne GmbH, Frickenhausen, Germany) at an initial density of 2,000 cells/cm2. All 4 biological samples were processed in a single color microarray design

将60%经绿色荧光蛋白（GFP）标记的人间充质干细胞（MSCGFP）与40%经mCherry标记的NIH:OVCAR-3肿瘤细胞进行共培养，使用MSC培养基在直径10 cm的细胞培养板（Greiner BioOne GmbH，德国弗里克森豪森）中进行孵育，初始接种密度为2000个细胞/平方厘米，培养时长最长达7天。所有4份生物学样本均采用单色微阵列设计完成处理。