Citrus_reticulata_sRNA

One year old seedlings of Shatangju mandarin and lemon were grafted with CLas-infected and CLas free buds. The department of Qingyuan National Citrus Seedling Breeding Base provided vigorous shatangju and lemon seedlings, which were grown as part of a certification program. Buds from disease-free, healthy citrus plants were used to mock inoculate the control plants. Each plant was kept in a greenhouse in the same temperature-controlled environment. Fluorescence quantitative PCR was used to detect whether the seedlings had been infected with CLas. Mock and CLas-infected shatangju and lemon plants,

将1年生砂糖橘（Shatangju）与柠檬实生苗分别嫁接携带CLas侵染的芽穗及未携带CLas的芽穗。清远国家柑橘种苗繁育基地提供了长势健壮的砂糖橘与柠檬实生苗，该批种苗均作为认证项目的一部分完成培育。采集无病健康柑橘植株的芽穗，对对照植株进行模拟接种。所有植株均置于同一控温环境的温室中培养。采用荧光定量PCR技术检测实生苗是否被CLas侵染。模拟接种组与CLas侵染组的砂糖橘及柠檬植株，