Life-history trade-offs and stress resistance in Drosophila melanogaster populations adapted to pathogenic bacterial infection

Evolution of increased immune defence is often limited by costs: correlated changes in other traits (viz. life-history traits) that otherwise reduce the fitness of the host organisms. Experimental evolution studies are useful for understanding the evolution of immune function, and correlated changes in other traits. We experimentally evolved replicate Drosophila melanogaster populations to better survive infection challenge with an entomopathogenic bacteria, Enterococcus faecalis. Within 35 generations of directional selection, selected populations showed marked increase in post-infection survival compared to ancestrally paired controls. We next measured various life-history traits of these populations. Our results show that the selected populations do not differ from control populations for larval development time and body weight at eclosion. No difference is also observed in case of fecundity and longevity (following the acute phase of infection), either when the flies were subjected ..., Life-history traits, and resistance to biotic and abiotic stressors, were measured for Drosophila melanogaster flies selected for improved post-infection survival against systemic infection with an entomopathogenic, Gram-positive bacteria, Enterococcus faecalis. The populations were named the EPN populations.
1. EPN populations: history and maintenance regime
The EPN populations was derived from ancestral Blue Ridge Baseline (BRB) populations (Singh et al., 2021). The BRB populations consist of 4 replicate outbred populations, originally established by hybridizing iso-female lines which had themselves been established by wild-caught flies, from Blue Ridge, USA, in the laboratory of Prof. Daniel Promislow (Singh et al., 2015). These populations are maintained in a 14-day discrete generation cycle, with a census size of about 2800 adults in every generation. These populations are maintained under standard lab conditions (12:12 light: dark cycle, 25 °C, 60% relative humidity) on banana-jag..., , # Life-history trade-offs and stress resistance in Drosophila melanogaster populations adapted to pathogenic bacterial infection

[https://doi.org/10.5061/dryad.1rn8pk14w](https://doi.org/10.5061/dryad.1rn8pk14w)

## Description of the data and file structure

**GENERAL INFORMATION**

1\. Title of Dataset: \"Life-history trade-offs and stress resistance in Drosophila melanogaster populations adapted to pathogenic bacterial infection\"

2\. Author Information

A. Principal Investigator Contact Information

Name: Prof. N. G. Prasad

Institution: Indian Institute of Science Education and Research, Mohali

Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India.

Email: [[e-mail]](mailto:[e-mail])

B. Associate or Co-investigator Contact Information

Name: Aparajita Singh

Institution: Indian Institute of Science Education and Research, Mohali

Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India.

Emai...

免疫防御增强的进化往往受限于代价：其他性状（即生活史性状，life-history traits）的相关变化会降低宿主生物的适合度。实验进化研究有助于理解免疫功能的进化及其他性状的相关变化。我们通过实验进化多组重复的黑腹果蝇（Drosophila melanogaster）种群，以提高其在昆虫病原细菌粪肠球菌（Enterococcus faecalis）感染胁迫下的存活率。经过35代定向选择后，与祖先配对的对照组相比，所选种群的感染后存活率显著提高。随后，我们测量了这些种群的多种生活史性状。结果显示，所选种群与对照种群在幼虫发育时间和羽化时体重方面无显著差异；在繁殖力和寿命（感染急性期后）方面亦未观察到差异，无论果蝇是否受到...。我们对经选择后在昆虫病原革兰氏阳性细菌粪肠球菌系统性感染中感染后存活率提高的黑腹果蝇，测量了其生活史性状及对生物和非生物胁迫的抗性。这些种群被命名为EPN种群。

1. EPN种群：历史与维持方案
EPN种群源自祖先蓝岭基线（Blue Ridge Baseline，BRB）种群（Singh等，2021）。BRB种群包含4组重复的远交种群，最初由Daniel Promislow教授实验室通过杂交单雌系建立，而这些单雌系则来自美国蓝岭地区的野生捕获果蝇（Singh等，2015）。这些种群以14天为一个不连续世代周期维持，每代成虫数量约为2800只。它们在标准实验室条件下（12:12光暗周期、25°C、60%相对湿度）于香蕉-jag...培养基上培养..., # 适应病原细菌感染的黑腹果蝇种群的生活史权衡与胁迫抗性

[https://doi.org/10.5061/dryad.1rn8pk14w]

## 数据与文件结构描述

**通用信息**
1. 数据集标题：《适应病原细菌感染的黑腹果蝇种群的生活史权衡与胁迫抗性》
2. 作者信息
A. 首席研究员联系方式
姓名：N. G. Prasad教授
机构：印度科学教育与研究学院莫哈里分校
地址：印度旁遮普邦SAS Nagar知识城81区IISER莫哈里分校，邮编140306
邮箱：[[e-mail]]
B. 副研究员或合作研究员联系方式
姓名：Aparajita Singh
机构：印度科学教育与研究学院莫哈里分校
地址：印度旁遮普邦SAS Nagar知识城81区IISER莫哈里分校，邮编140306
邮箱...