Trajectories, clustering analysis, input files and paramters for Influence of an RNA template on DNA nonenzymatic primer extension

A detailed structural investigation was conducted on DNA self-replication using an RNA template with phosphoroimidazole activation. The study reveals a clear dependence between helix type and the efficiency of nonenzymatic primer extension. Specifically, the RNA template imposes A-type helicity on the hybrid helix, enhancing the efficiency of primer extension.

本研究针对采用磷酰咪唑活化（phosphoroimidazole activation）的RNA模板（RNA template），开展DNA自我复制（DNA self-replication）的详细结构探究。研究清晰揭示了螺旋类型与非酶促引物延伸（nonenzymatic primer extension）效率之间的明确依赖关系。具体而言，RNA模板可使杂合螺旋（hybrid helix）呈现A型螺旋性（A-type helicity），进而提升引物延伸的反应效率。