CotA-mediated transcriptional responses to carbon source diversity in Aspergillus fumigatus

Aspergillus fumigatus CotA is a conserved, Nuclear Dbf2-Related (NDR) kinase that is required for growth in conditions where non-carbohydrates are provided as the sole carbon source. The activation of broad-scale transcriptional regulatory programs, like the carbon catabolite repression system, are major mechanisms through which microbes conserve energy and ensure optimal growth in response diverse nutritional environments. This study aimed to characterize the global transcriptional response of A. fumigatus when cultured in glucose, acetate or ethanol using both wild type (strain CEA10) and mutant (cotA gene disruption) strains to test the hypothesis that CotA regulates cellular transcriptional reprogramming in response carbon diversity.

烟曲霉（Aspergillus fumigatus）CotA是一种保守的核Dbf2相关（NDR）激酶，在以非碳水化合物作为唯一碳源的培养条件下，其为菌体生长所必需。诸如碳分解代谢物阻遏系统（carbon catabolite repression system）在内的大规模转录调控程序的激活，是微生物储存能量并在多样化营养环境中保障最优生长的核心机制。本研究以野生型菌株CEA10与cotA基因敲除突变株为材料，分析烟曲霉在葡萄糖、乙酸盐或乙醇中培养时的全局转录响应，以验证CotA可调控细胞响应碳源多样性的转录重编程这一假说。