RNA-seq revealed counter effect of chitosan against Botrytis cinerea on grape berries

Plants have great potential to protect against pathogens and other abiotic stress, and thus the interaction between signaling networks of defense is incredible. This can be activated and express with the application of growth elicitors and antimicrobial natural compounds. In the present study we used natural polysaccharide, Chitosan (15kDa) against Grey mold in two grape varieties (Shine-muscat and Kyoho). The finding depicted that the interaction of DEG and KEGG in control and treated samples of grapevine, which provides the evidence for selection of gray mold related defense genes and chitosan for subsequent grapevine production/postharvest programs. Two Grape verities (Shine-muscat and Kyoho) treated with resistance inducers chitosan, the physiological changes in the grape berry were analyzed at 80-90 % physiologically maturities, with commercial grade chitosan, formulations were applied by foliar spray: 15% (w/v) chitosan (15 kDa) (Fig.1). To prepare formulations, we used distilled water as control. Each berry of grapy is treated as one replication and each replication is repeated together and randomized 3 times. One day later, Botrytis cinerea spores with 104 /μl concentration was used. The gray mold fungus was grown on grape berries at 25 °C, and 95 % relative humidity.

植物在抵御病原菌与其他非生物胁迫方面具备极高潜力，其防御信号网络之间的互作机制尤为精妙。该防御通路可通过施加生长激发子（growth elicitors）与抗菌天然化合物得以激活并启动相关基因表达。本研究采用天然多糖壳聚糖（Chitosan，15kDa）防治两种葡萄品种（阳光玫瑰Shine-Muscat与巨峰Kyoho）的灰霉病（Grey mold）。研究结果揭示了葡萄对照组与处理组样本中差异表达基因（DEG）与京都基因与基因组百科全书（KEGG）富集通路的互作关系，为筛选灰霉病相关防御基因及壳聚糖应用于后续葡萄生产/采后处理方案提供了理论依据。本研究以两种葡萄品种（阳光玫瑰Shine-Muscat与巨峰Kyoho）为材料，通过抗性诱导剂壳聚糖进行处理，在葡萄浆果生理成熟度达80%-90%时分析其生理变化：采用商业级壳聚糖配置成15%（w/v）的15kDa壳聚糖制剂，通过叶面喷施方式施药（见图1），制剂配置以蒸馏水作为空白对照。每个葡萄浆果视为一个生物学重复，每组重复开展三次独立实验并进行随机化处理。处理1天后，接种浓度为10^4个/μl的灰葡萄孢（Botrytis cinerea）孢子悬浮液。将接种后的葡萄浆果置于25℃、相对湿度95%的条件下培养。