Quantitative Stability of Hematopoietic Stem and Progenitor Cell Clonal Output in Transplanted Rhesus Macaques

Following total body irradiation, four rhesus macaques were transplanted with lentivirally transduced HSPCs containing unique 35 base-pair barcode sequences in the provirus insertions. Blood was extracted from macaques at time points up through 4 years post-transplant and sorted into cell subsets; targeted DNA sequencing of the barcoded region then allowed for quantification of barcodes in cell subsets.

接受全身照射（total body irradiation）后，四只恒河猴（rhesus macaques）接受了慢病毒转导的造血干细胞及祖细胞（hematopoietic stem and progenitor cells, HSPCs）移植，此类细胞的前病毒整合位点中携带有独特的35碱基对条形码序列（barcode sequences）。研究人员在移植后长达4年的多个时间点采集恒河猴血液，并将血液分选至不同细胞亚群中；随后对带条形码的区域开展靶向DNA测序，即可实现各细胞亚群内条形码的定量检测。