KRYPTONITE shapes TAD-like boundaries through the control of H3K9ac [ATAC-Seq]

Employing high-throughput sequencing and advanced microscopy techniques, we explored twenty-six histone modifications and Pol II distribution landscape in tomato (Solanum lycopersicum). Our findings revealed a comprehensive epigenetic landscape, uncovering distinct chromatin states associated with heterochromatin formation and gene silencing, allowing for the classification of genomic regions. Comparative analysis of twenty-six histone modifications and Pol II ChIP-seq data for tomato M82 wild type, HiC and Comparative analysis of ChIP-seq, ATACseq, RNAseq for tomato M82 wild type and Kyp mutant

本研究借助高通量测序与先进显微技术，对番茄（Solanum lycopersicum）中的26种组蛋白修饰及RNA聚合酶II（Pol II）的分布图谱展开了系统性探究。研究结果揭示了一套完整的表观调控图谱，发现了与异染色质形成及基因沉默相关的特异性染色质状态，可用于基因组区域的分类鉴定。此外，本研究开展了两项比较分析：一是针对番茄M82野生型的26种组蛋白修饰与Pol II的染色质免疫沉淀测序（ChIP-seq）数据及HiC数据的比较分析；二是针对番茄M82野生型与Kyp突变体的染色质免疫沉淀测序（ChIP-seq）、转座酶可及性测序（ATACseq）与转录组测序（RNAseq）的比较分析。