Effect of Lavandula dentata extract on Ovalbumin-induced Asthma in Male Guinea Pigs

Abstract Asthma is an inflammatory disease of the lungs, and it causes oxidative stress. Lavandula dentata is an aromatic herb with anti-oxidative and anti-inflammatory activities. This study examined the activity of L. dentata extract on a guinea pig model of asthma. Adult males were divided into five groups: First group was control, second was asthma model induced by OVA, third was treated with L. dentata extract orally (300 mg/kg) for 21 days; the fourth was an asthma model with L. dentata extract (300 mg/kg) and fifth was treated with Tween 80 for 21 days. OVA treatment increased IgE, triglycerides, total cholesterol, glucose levels in serum, WBC count in blood and MDA in lungs. Also, OVA reduced SOD activity, GSH content in lungs, and GGT activity in serum (p<0.05). L. dentata extract treatment in asthma model reduced elevated IgE, triglycerides, total cholesterol, glucose levels in serum, and MDA in lungs (p<0.05), while it increased GSH content in lungs (p<0.05). These results suggest the possibility that L . dentata extract can exert suppressive effects on asthma, and may provide evidence that it is a useful agent for the treatment of allergic airway disease, it also limits oxidative stress induced by OVA. L. dentata extract appears to have hypolipidemic and hypoglycemic activities.

摘要 哮喘是一种肺部炎症性疾病，可引发氧化应激。齿叶薰衣草（Lavandula dentata）是一种兼具抗氧化与抗炎活性的芳香草本植物。本研究针对哮喘豚鼠模型，考察了齿叶薰衣草提取物的相关活性。将成年雄性豚鼠分为五组：第一组为对照组；第二组为卵清蛋白（OVA）诱导的哮喘模型组；第三组为口服齿叶薰衣草提取物（300 mg/kg）干预21天的组别；第四组为哮喘模型联合齿叶薰衣草提取物（300 mg/kg）干预组；第五组为以吐温80（Tween 80）干预21天的组别。卵清蛋白造模可使豚鼠血清中免疫球蛋白E（IgE）、甘油三酯、总胆固醇、葡萄糖水平，血液白细胞（WBC）计数以及肺部丙二醛（MDA）含量升高；同时降低肺部超氧化物歧化酶（SOD）活性、谷胱甘肽（GSH）含量，以及血清γ-谷氨酰转移酶（GGT）活性（p<0.05）。经齿叶薰衣草提取物干预的哮喘模型组，其升高的血清IgE、甘油三酯、总胆固醇、葡萄糖水平以及肺部MDA含量均显著降低（p<0.05），同时肺部GSH含量显著升高（p<0.05）。上述结果表明，齿叶薰衣草提取物可对哮喘发挥抑制作用，为其作为变应性气道疾病治疗的有效制剂提供了实验依据，同时可缓解卵清蛋白诱导的氧化应激。此外，齿叶薰衣草提取物还表现出调脂与降血糖活性。