Association between deltamethrin resistance, P. falciparum infection and the Vgsc-1014S resistance mutation in A. gambiae highlights the importance of resistance markers

WHO standard methods were used to characterize susceptibility of wild female mosquitoes to 0.05 % deltamethrin. PCR-based molecular diagnostics were used to identify mosquitoes to species and to genotype at the Vgsc-L1014S locus. ELISAs were used to detect the presence of P. falciparum sporozoites and for blood meal identification. (MapVEu VBP0000170)

本研究采用世界卫生组织（WHO）标准方法，对野生雌性蚊虫对0.05%溴氰菊酯的敏感性进行表征。基于聚合酶链式反应（PCR）的分子诊断技术被用于蚊虫物种鉴定，以及Vgsc-L1014S位点的基因分型。酶联免疫吸附试验（ELISA）被用于检测恶性疟原虫（P. falciparum）子孢子的存在，并完成血餐鉴定。(MapVEu VBP0000170)