Metagenome assembled genomes co_2-Bin_23_1.fa

Co-assemblies were binned with CONCOCT within anvi’o v5.1 by clustering scaffolds 2500 bp or longer into metagenome assembled genomes (MAGs) and manually refining them within the anvi’o platform. Furthermore, dRep v2.3.2 was used to dereplicate MAGs and obtain representative genomes with flags ‘-comp 50 -con 10’ and default values.

本研究中的共组装结果借助CONCOCT工具并在anvi’o v5.1版本平台内完成分箱：将长度不小于2500 bp的支架序列（scaffold）聚类为宏基因组组装基因组（Metagenome Assembled Genomes，简称MAGs），并在anvi’o平台内对其进行人工精修。此外，采用dRep v2.3.2工具对MAGs进行去冗余处理，通过设置参数‘-comp 50 -con 10’并采用其余默认参数，以获取代表性基因组。