Editing at AAVS1 locus by CRISPR/Cas9 vectors incorporating U6 or 7SK promoters

The editing efficiency of LCv2-U6 and LCv2-7SK targeting AAVS1 were compared through transduction of HEK293 cells. Genomic DNA was isolated and the AAVS1 locus amplified and sequenced.

本研究通过转导HEK293细胞，对比LCv2-U6与LCv2-7SK靶向AAVS1位点的基因编辑效率。随后提取基因组DNA，对AAVS1位点进行扩增并测序。