Effects of plantain on transcriptional profile of liver in obese rats. Effects of plantain on transcriptional profile of liver in obese rats

We explored the mechanisms underlying the anti-obesity effect of plantain. Plantain flour was used to feed obese rats fed a high-fat diet. Six groups were set, viz., the normal control (NC), obese control (OC), low-dose (LD), middle-dose (MD), high-dose (HD), and positive control (PC). The differentially expressed genes in the liver, as well as the lipid and glucose metabolic pathways were investigated. Transcriptome analyses showed that the expression of the PPARα, CPT1A, AMPK, and PGC1α genes was upregulated in the plantain intervention groups. Conclusion: Some key genes in fatty acid and sugar metabolic pathways were regulated by the plantain diet, which probably led to the observed fat and weight loss effects. Overall design: liver mRNA profiles of obese rats and obese rats treated with plantain were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000

本研究探究了车前草抗肥胖作用的潜在分子机制。将车前草粉用于饲喂经高脂膳食造模的肥胖大鼠，共设置6个实验组，即正常对照组（normal control, NC）、肥胖模型对照组（obese control, OC）、低剂量组（low-dose, LD）、中剂量组（middle-dose, MD）、高剂量组（high-dose, HD）以及阳性对照组（positive control, PC）。本研究对大鼠肝脏组织中的差异表达基因与脂质、葡萄糖代谢通路展开了分析。转录组测序分析结果显示，车前草干预组中PPARα、CPT1A、AMPK及PGC1α基因的表达均显著上调。研究结论表明，车前草膳食可调控脂肪酸与糖类代谢通路中的关键基因，这可能是其发挥观测到的减脂与减重效应的潜在机制。实验整体设计：采用Illumina NovaSeq 6000平台开展深度测序，对肥胖大鼠及经车前草干预的肥胖大鼠的肝脏mRNA表达谱进行三次生物学重复检测。