Genome-wide DNA methylation pattern in whole blood associated with primary intracerebral hemorrhage. Genome-wide DNA methylation pattern in whole blood associated with primary intracerebral hemorrhage

To reveal the role of DNA methylation in peripheral blood from primary intracerebral hemorrhage(ICH) ,DNAs from 30 ICH patients and 34 matched controls were analyzed by methylation microarray. In total, we identified 1377 hypomethylated and 153 hypermethylated differentially methylated positions (DMPs) in peripheral blood from ICH patients compared to healthy controls. Overall design: Peripheral blood samples from 30 patients with ICH and 34 matched controls were analyzed by Illumina Infinium HumanMethylation850K BeadChip.

为揭示原发性脑出血（primary intracerebral hemorrhage, ICH）患者外周血中DNA甲基化的相关作用，本研究对30例ICH患者与34例匹配对照的基因组DNA样本进行了甲基化微阵列分析。相较于健康对照人群，本研究在ICH患者的外周血中共鉴定出1377个低甲基化差异甲基化位点（differentially methylated positions, DMPs）与153个高甲基化差异甲基化位点。
实验整体设计：采用Illumina Infinium HumanMethylation850K BeadChip芯片，对30例ICH患者及34例匹配对照的外周血样本开展检测分析。