Effect of chronic cold on gene expression in inguinal white adipose tissue.

Excess weight is associated with an increased risk of insulin resistance and type 2 diabetes. Activating thermogenic mechanisms is a potentially powerful means to reduce excess weight and achieve metabolic benefits. Recent studies show that cold stimuli trigger the conversion of white fat into beige fat, which can mediate non-shivering thermogenesis. The purpose of this study is to determine effect of chronic cold exposure on gene expression in iWAT. To this end, we performed RNA-sequencing of iWAT from mice at 30 °C or 7 °C for 3 weeks and identified significantly regulated genes under chronic cold in iWAT. Overall design: Transcriptome profiles of iWAT of 9-week-old male mice (C57BL/6J) housed at 30 °C or 7 °C for 3 weeks.

超重与胰岛素抵抗及2型糖尿病的发病风险升高密切相关。激活产热机制是减轻超重、获得代谢益处的潜在有效手段。既往研究表明，寒冷刺激可诱导白色脂肪向米色脂肪转化，后者可介导非战栗产热过程。本研究旨在探究慢性寒冷暴露对腹股沟白色脂肪组织（inguinal white adipose tissue, iWAT）基因表达的影响。为此，我们对分别于30℃或7℃环境中饲养3周的小鼠的iWAT进行RNA测序（RNA-sequencing），并筛选出慢性寒冷暴露下iWAT中显著差异表达的基因。实验整体设计：以分别于30℃或7℃环境中饲养3周的9周龄雄性C57BL/6J小鼠的iWAT为样本，获取其转录组表达谱。