Expression data from epidermal human skin (stem cells, transient amplifying cells, differentiated cells)

Performing global gene expression profiling for different types of keratinocytes colonies can provide important information that might help in understanding the biology and ontogeny of epidermal skin stem cells. We used microarrays to detail the global gene expression profiling for different types of keratinocytes colonies including Holoclones (stem cells), Meroclones (transient amplifying cells), and Paraclones (differentiated cells) . A total of 100 colonies per foreskin sample (n=6) were analyzed. The classification was based on the percentage of aborted colonies (< 3 mm2 in diameter); When 0–5% of colonies were aborted the clone was scored as holoclone which represents the stem cells. When more than 95% of the colonies were aborted, the clone was classified as paraclone which represents the differentiated cells. When more than 5%, but less than 95% of the colonies were aborted, the clone was classified as meroclone which represent transient amplifying cells

对不同类型角质形成细胞集落开展全局基因表达谱分析，可为解析表皮皮肤干细胞的生物学特性及个体发育起源提供关键参考信息。本研究采用微阵列（microarrays）技术，对包含全克隆（Holoclones，干细胞）、半克隆（Meroclones，瞬时扩增细胞）与旁克隆（Paraclones，分化细胞）在内的多种角质形成细胞集落的全局基因表达谱进行了详细分析。共分析了6份包皮样本，每份样本对应100个集落（n=6）。集落分类依据为败育集落的占比（败育集落直径<3 mm²）：若败育集落占比为0–5%，则该克隆被鉴定为全克隆，代表干细胞；若败育集落占比超过95%，则该克隆被归类为旁克隆，代表分化细胞；若败育集落占比介于5%至95%之间，则该克隆被归类为半克隆，代表瞬时扩增细胞。