Clinical significance of MSP-RON signaling based on mRNA-microRNA integration analyses in acute severe ischemic stroke patients (micro RNA data). Clinical significance of MSP-RON signaling based on mRNA-microRNA integration analyses in acute severe ischemic stroke patients (micro RNA data)

The roles of mRNA and microRNA (miRNA) are widely known in many diseases including acute ischemic stroke. About 60 % of all human messenger RNAs (mRNAs) are regulated by microRNAs. Integration analysis using mRNA and miRNA are important to elucidate pathogenesis. But the contribution of mRNA and miRNA, especially miRNA targeted mRNA, related with severity of acute ischemic stroke is not remain understood. To clarify the pathway related with the severity of acute ischemic stroke, we examined mRNA and miRNA integration analysis targeted for acute ischemic stroke. Overall design: We corrected RNA extracted from whole blood of 6 acute minor ischemic stroke patients (National Institutes of Health Stroke Scale [NIHSS] < 8) and 6 acute severe ischemic stroke patients (NIHSS ≥ 8) on admission. mRNA and miRNA were measured by RNA sequencing.

mRNA与微小RNA（microRNA, miRNA）在包括急性缺血性脑卒中在内的多种疾病中的作用已被广泛认知。人类体内约60%的信使RNA（messenger RNA, mRNA）受微小RNA调控。利用mRNA与miRNA开展整合分析，对于阐明疾病发病机制具有重要意义。但目前关于mRNA与miRNA——尤其是miRNA靶向调控的mRNA——与急性缺血性脑卒中严重程度的关联贡献，尚未完全明确。为明确与急性缺血性脑卒中严重程度相关的通路，本研究针对急性缺血性脑卒中开展了mRNA与miRNA整合分析。整体实验设计：本研究采集了6例轻度急性缺血性脑卒中患者（入院时美国国立卫生研究院卒中量表[National Institutes of Health Stroke Scale, NIHSS]评分<8）与6例重度急性缺血性脑卒中患者（NIHSS评分≥8）的全血样本，提取RNA并进行校正后，通过RNA测序对mRNA与miRNA进行了检测。