Day23_exon3lines_Rep2

Haploinsufficiency of transcriptional regulators causes human congenital heart disease (CHD), predicting gene regulatory network (GRN) imbalances. Here, we define transcriptional responses to reduced transcription factor dosage in human iPSCs heterozygous or homozygous for loss of the CHD gene TBX5. Single cell RNA sequencing of day 23 cells from exon3 lines (WTC11, TBX5Het and TBX5Hom) - biological replicate 2 Processed data are on the GSE137873 Series record.

转录调控因子的单倍剂量不足 (haploinsufficiency) 可引发人类先天性心脏病 (congenital heart disease, CHD)，该现象提示基因调控网络 (gene regulatory network, GRN) 存在失衡。本研究针对CHD致病基因TBX5功能缺失的杂合或纯合人类诱导多能干细胞 (human induced pluripotent stem cells, iPSCs)，明确了转录因子剂量降低所介导的转录应答反应。我们对来自外显子3编辑细胞系（WTC11、TBX5杂合缺失型 (TBX5Het) 与TBX5纯合缺失型 (TBX5Hom)）的第23天细胞开展了单细胞RNA测序 (single cell RNA sequencing)，该实验为第2个生物学重复。处理后的数据已收录于GSE137873系列数据集记录。