Knockdown of the long noncoding RNA PURPL induces apoptosis and sensitizes liver cancer cells to doxorubicin

Liver cancer was the fourth leading cause of cancer-related deaths worldwide in 2018, of which Hepatocellular carcinoma accounts for 75-85% of all liver cancers and is therefore the most common type of liver cancer. In this study, total RNA were extracted from sections of formalin-fixed paraffin embedded (FFPE) archival human liver biopsies from 24 patients, including ten HCV-associated HCC (HCV-HCC), ten virus-unrelated HCC (VU-HCC) and four normal liver patients. By comparing the expression profiles of HCV-HCC and VU-HCC to that of the normal liver biopsies respectively, 18 lncRNAs were identified to be differentially expressed. Total RNA human liver tissue was sequenced from grouped FFPE archival samples.

2018年，肝癌为全球第四大癌症相关死亡病因，其中肝细胞癌（hepatocellular carcinoma）占所有肝癌病例的75%~85%，是最为常见的肝癌亚型。本研究从24名受试者的福尔马林固定石蜡包埋（formalin-fixed paraffin embedded, FFPE）存档人类肝脏活检组织切片中提取总RNA，样本涵盖10例丙型肝炎病毒相关性肝细胞癌（HCV-associated HCC, HCV-HCC）、10例病毒无关性肝细胞癌（virus-unrelated HCC, VU-HCC）以及4例正常肝脏对照样本。通过分别对比HCV-HCC、VU-HCC与正常肝脏活检组织的基因表达谱，本研究共鉴定出18个差异表达的长链非编码RNA（long non-coding RNA, lncRNA）。本研究针对分组后的FFPE存档人类肝脏组织样本，完成了总RNA测序。