MiRNA expression data of distal colon mucosa from young and old mice

The disruption of the mucosal barrier of the digestive tract is a common pathological change in the elderly, which often causes inflammatory bowel disease among old people.Given that microRNA (miRNA) molecules are dysregulated in many diseases, the miRNA expression in young and old normal distal colon mucosa in mice was determined by high-throughput analysis. Three 2-month-old mice and 3 24-month-old were enrolled in this study. We used sequencing analysis to identify miRNA expression in the distal colon mucosa. Male C57BL/6 mice divided into two groups according to age, i.e., 2 and 24 month old groups (n =3 per group). All mice were housed in a controlled environment under a 12 h light/dark cycle at 22 ± 2 ℃, 55 ± 5% humidity with access to food and water ad libitum and were sacrificed by appropriate anesthesia and/or cervical dislocation prior to the sample collection. The distal colon tissues were surgically and aseptically removed from the mice. After collection, the samples were stored at -80 °C.

消化道黏膜屏障破坏是老年群体常见的病理改变，常引发老年人炎症性肠病。鉴于微RNA（microRNA, miRNA）分子在多种疾病中存在表达失调情况，本研究通过高通量分析检测了小鼠年轻与年老正常远端结肠黏膜中的miRNA表达水平。本研究共纳入3只2月龄小鼠与3只24月龄小鼠，采用测序分析鉴定远端结肠黏膜中的miRNA表达。实验所用小鼠均为雄性C57BL/6品系，按年龄分为两组，即2月龄组与24月龄组，每组各3只（n=3）。所有小鼠均饲养于可控环境中：光照与黑暗循环周期为12小时，环境温度维持在22±2℃，相对湿度为55±5%，可自由采食饮水；样本采集前，通过适当麻醉及/或颈椎脱臼法处死小鼠。随后通过外科无菌操作获取小鼠远端结肠组织，样本采集完成后即刻保存于-80℃环境中。