Gene expression profiling of the prostate biopsy samples (cancer and adjacent normal tissues) from African American prostate cancer patients. Homo sapiens

Prostate cancer (PCa) tends to be more aggressive and lethal in African Americans (AA) compared to European Americans (EA). To further understand the biological factors accounting for the PCa disparities observed in AA and EA patients, we performed gene profiling using Affymetrix human exon 1.0 ST arrays to identify the differentially expressed genes beween AA cancer and patient matched normal tissues. Overall design: 40 prostate biopsy specimens (tumor and adjacent normal tissues) were collected from 20 African American prostate cancer patients. RNA samples, purified from the collected biopy specimens, were process and applied to Affymetrix human exon ST 1.0 arrays. Array data was normalized, batch-corrected and analyzed (2-way ANOVA) using Partek Genomics Suite program.

与欧洲裔美国人（European Americans, EA）相比，非洲裔美国人（African Americans, AA）所患前列腺癌（Prostate Cancer, PCa）通常具有更强的侵袭性与更高的致死率。为进一步阐明导致非洲裔与欧洲裔前列腺癌患者间预后差异的生物学因素，本研究采用Affymetrix人类外显子组1.0 ST芯片进行基因表达谱分析，以鉴定非洲裔美国人前列腺癌组织与其自体配对正常组织间的差异表达基因。
实验整体设计：从20名非洲裔前列腺癌患者体内采集了40份前列腺活检标本，涵盖肿瘤组织及其自体配对的癌旁正常组织。从上述活检标本中纯化得到的RNA样本经处理后，被应用于Affymetrix人类外显子组1.0 ST芯片检测。芯片数据采用Partek基因组学套件（Partek Genomics Suite）进行归一化、批次校正及双向方差分析（2-way ANOVA）。