Gene expression data from somites of E9.5 mouse embryos. Mus musculus

We used microarrays to identify Pax3 targets during myogenesis in the mouse embryo Overall design: Mouse embryos were genotyped Pax3GFP/+ or Pax3PAX3-FKHR/GFP and dissected at E9.5 under a fluorescent binocular. Somites were dissected from the interlimb region and the more hypaxial domain separated from the neural tube and the epaxial extremity of the somites. GFP positive cells were then sorted by flow cytometry before RNA extraction and hybridization on Affymetrix microarrays. We also sorted GFP negative cells.

本研究利用微阵列（microarrays）技术，在小鼠胚胎肌发生过程中筛选Pax3的靶基因。
实验设计概况：对基因型为Pax3GFP/+或Pax3PAX3-FKHR/GFP的小鼠胚胎，于胚胎发育第9.5天（E9.5）在荧光体视显微镜下进行解剖操作。从肢间区域分离体节，并将体节的轴下域与神经管及体节的轴上极分离。随后通过流式细胞术（flow cytometry）分选绿色荧光蛋白（GFP）阳性细胞，提取RNA后在Affymetrix微阵列上完成杂交；同时同步分选GFP阴性细胞。