Effect of loss of lymphocytes in Rag2-/- mice on gene expression of glial cells. Effect of loss of lymphocytes in Rag2-/- mice on gene expression of glial cells

To investigate the influence of peripheral lymphocytes on cells in the brain, we purified four major classes of brain cells and performed RNA sequencing from mice lacking lymphocytes Overall design: Glial cells were purified from the cerebral cortex of adult Rag2-/- mice and their immune-competent littermates.

为探究外周淋巴细胞（peripheral lymphocytes）对脑内细胞的影响，我们分离纯化了四类主要脑细胞，并对淋巴细胞缺失型小鼠的此类脑细胞开展了RNA测序（RNA sequencing）实验。整体实验设计：我们从成年Rag2-/-小鼠及其免疫健全的同窝对照小鼠的大脑皮层中分离纯化了胶质细胞（glial cells）。