Gene expression modulation assessed by quantitative PCR in fibroblasts of reconstructed skins exposed to UVA1.

Reconstructed skins were exposed to 20 or 40 J/cm2 UVA1 and recovered 2, 6 or 24 hours later. Expression of genes found modulated in the microarray study and/or representative of main functional families was analyzed by quantitative PCR in fibroblasts of reconstructed skin. Ratios of modulation induced by UVA1 exposure were calculated for each condition as the ratio of mean mRNA amount in UVA1 exposed samples to mean mRNA amount in sham exposed control samples (columns 2 to 7). Bold text indicate mean ratio values with significant differences between UVA1 exposed and control samples (p

将重建皮肤暴露于20或40 J/cm²的UVA1（长波紫外线1），并分别于照射后2、6或24小时收取样本。通过定量PCR（quantitative PCR）检测重建皮肤成纤维细胞中，基因芯片（microarray）研究中发现的差异表达基因，以及主要功能家族代表性基因的表达水平。针对各实验条件，计算UVA1照射诱导的调控倍数：即UVA1照射组样本的平均mRNA量与假照射对照组样本的平均mRNA量的比值（对应表格第2至7列）。粗体文本表示UVA1照射组与对照组间存在显著差异的平均调控倍数（p