RNAseq of A/J mice spiral ganglion derived neurospheres vs differentiated auditory neurons. RNAseq of A/J mice spiral ganglion derived neurospheres vs differentiated auditory neurons

Sphere-forming progenitor cells can be isolated from the fetal and adult mammalian inner ear and give rise to inner ear specific cell types in vitro. Here, we provide phenotypical and functional characterization of a new pool of auditory progenitors as sustainable source for sphere-derived auditory neurons. The so-called phoenix auditory neuroprogenitors, isolated from the A/J mouse spiral ganglion, exhibit nearly unlimited intrinsic self-renewal properties (beyond 40 passages). At any passage, phoenix spheres can be efficiently differentiated by withdrawing growth factors into mature spiral ganglion cells, expressing both neurons and glial cells phenotypic markers and exhibiting similar functional properties as mouse spiral ganglion explants and human sphere-derived spiral ganglion cells. The present dataset includes RNAseq-based transcriptome analysis of phoenix auditory neurons following 7 days of differentiation. mRNA levels in differentiated cells are expressed relatively to neuroprogenitor spheres of equivalent passage (paired samples at passage 12, 21 and 36)

可从胎儿期及成年哺乳动物内耳中分离得到成球祖细胞（sphere-forming progenitor cells），该类细胞在体外可分化产生内耳特异性细胞类型。本研究针对一类新型听觉祖细胞群体开展了表型与功能特征表征，该细胞可作为成球来源听觉神经元的可持续来源。这类被命名为凤凰型听觉神经祖细胞（phoenix auditory neuroprogenitors）的细胞分离自A/J小鼠螺旋神经节，具备近乎无限的内在自我更新能力（传代次数可超过40次）。在任意传代阶段，均可通过撤除生长因子，高效诱导凤凰球分化为成熟螺旋神经节细胞，此类细胞可同时表达神经元与胶质细胞的表型标志物，且功能特性与小鼠螺旋神经节外植体及人类成球来源螺旋神经节细胞相近。本数据集包含分化培养7天后的凤凰型听觉神经元的基于RNA测序（RNAseq）的转录组分析数据。分化细胞的mRNA表达水平以对应传代的神经祖细胞球作为参照（配对样本取自传代12、21及36代）。