Broad transcriptional firing represses bactericidal activity in human airway neutrophils [10hours_transmigrated_CFASN_Neutrophils]

In this model, naïve blood neutrophils are migrated through a small airway epithelium grown at air-liquid interface toward CF airway fluid supernatant (CFASN, which corresponds to sputum sequentially centrifuged to remove cells and bacteria) or leukotriene B4 (LTB4, transmigration control), placed apically. To investigate a potential causal relationship between de novo transcription and neutrophil fate acquisition, we transmigrated neutrophils in the model for 10 hours toward CFASN or LTB4.

本实验模型中，将经气液界面（air-liquid interface）培养的小气道上皮细胞的顶端添加囊性纤维化气道上清液（CF airway fluid supernatant, CFASN，即经连续离心去除细胞与细菌的痰液）或白三烯B4（leukotriene B4, LTB4，趋化迁移对照），随后使未致敏血液中性粒细胞穿过该上皮层向上述趋化因子进行迁移。为探究从头转录与中性粒细胞命运获得之间的潜在因果关系，我们在该模型中让中性粒细胞向CFASN或LTB4进行10小时的趋化迁移。