Frontal cortex of male piglets housed in barren and environmentally enriched conditions. Frontal cortex of pigs in enriched environments

This dataset comprises RNA-sequencing of the frontal cortex of male piglets housed either in barren (control) pens or pens with additional environmental enrichment. 8 weaner pigs (1:1 male:female ratio) were housed in each of 6 enriched pens, with litter, weight and sex-matched controls in the barren pens, i.e. a total of 24 pigs in each of the barren and enriched conditions. At 56 days of age (+/- 3 days) one male per pen was sedated at 1 hour from the introduction of the enrichment stimulus, and one male per pen at 4 hours post enrichment stimulus. From this total set of samples (6x barren 1 hour, 6x enrichment 1 hour, 6x barren 4 hour, 6x enrichment 4 hour), polyA+ RNA-seq libraries were prepared by Edinburgh Genomics and >30 million stand-specific 75bp paired-end reads were sequenced per sample, using the Illumina HiSeq 4000 sequencing platform. These data are released under Fort Lauderdale principles, as confirmed in the Toronto Statement (Toronto International Data Release Workshop. Birney et al. 2009. Pre-publication data sharing. Nature 461:168-170). Data producers reserve the right to make the first publication of a global analysis of this data. If you are unsure if you are allowed to publish on this dataset, please contact [e-mail] or sarah.brown.ed.ac.uk to enquire.

本数据集涵盖饲养于无额外环境丰容的对照栏（barren pens）与带有额外环境丰容的饲养栏的雄性仔猪前额叶皮层的RNA测序数据。6个环境丰容栏各饲养8头断奶仔猪（雌雄比1:1），贫瘠对照栏则饲养与丰容栏仔猪窝别、体重及性别均匹配的对照仔猪，因此贫瘠环境组与丰容环境组各共计24头仔猪。在56日龄（±3日）时，于环境丰容刺激引入后1小时，每栏选取1头雄性仔猪进行镇静；并于丰容刺激后4小时，每栏再选取1头雄性仔猪实施镇静。该总样本集涵盖6个贫瘠组1小时样本、6个丰容组1小时样本、6个贫瘠组4小时样本及6个丰容组4小时样本。爱丁堡基因组学（Edinburgh Genomics）为该批样本构建了polyA+ RNA测序文库，并采用Illumina HiSeq 4000测序平台对每个样本进行测序，每个样本获得超过3000万条链特异性75bp双端读段。本数据集遵循劳德代尔原则（Fort Lauderdale principles）发布，该原则已在《多伦多声明》（Toronto Statement, Toronto International Data Release Workshop. Birney et al. 2009. Pre-publication data sharing. Nature 461:168-170）中得到确认。数据集制作者保留对本数据集开展全局分析并首次发表相关研究成果的权利。若您不确定是否可基于本数据集发表研究，请联系[e-mail]或sarah.brown.ed.ac.uk进行咨询。