The role of genetic diversity in the evolution and maintenance of environmentally-cued, male alternative reproductive tactics datasets.

In Stewart et al., 2019,"The role of genetic diversity in the evolution and maintenance of environmentally-cued, male alternative reproductive tactics", BMC Evolutionary Biology, we tested 16 nuclear simple sequence repeats (nSSRs) primer pairs designed for Rhizoglyphus robini, optimizing the primer pairs and concomitant PCR protocol for our own populations using Dreamtaq polymerase (Thermo Fisher Scientific). After protocol optimization, we found only 9 of the 16 nSSRs amplified well for our populations, of which 3 loci revealed fixation, and 6 demonstrating both clean/readable peaks and polymorphism across individuals. These 6 nSSRs were chosen for the genotyping of all remaining individuals. Here, the datasets generated and analysed in our study are presented.<br>

本研究参考Stewart等人2019年发表于《BMC进化生物学》（BMC Evolutionary Biology）的论文《环境调控的雄性替代繁殖策略的演化与维持中遗传多样性的作用》（"The role of genetic diversity in the evolution and maintenance of environmentally-cued, male alternative reproductive tactics"），针对罗宾根螨（Rhizoglyphus robini）设计的16对核简单序列重复（nuclear simple sequence repeats, nSSRs）引物进行了验证，并使用DreamTaq DNA聚合酶（赛默飞世尔科技，Thermo Fisher Scientific）对本研究种群的引物及配套聚合酶链式反应（PCR）体系进行了优化。经体系优化后，16对nSSRs引物中仅9对可在本研究种群中实现有效扩增；其中3个基因座呈现固定状态，剩余6个基因座既能得到清晰可读的峰型，又可在不同个体间展现多态性。最终选取这6对nSSRs引物用于所有剩余个体的基因分型。本文将呈现本研究中生成并分析的全部数据集。