Analysis of sex chromosomes effect on hybrid male sterility

Sex chromosomes and more particularely the X chromosomes are known to have a major effect on hybrid male sterility. In this experiment by making use of the reciprocal hybrids between D. simulans and D. sechellia, we are showing the effect of these chromosomes on gene expression in male hybrids Keywords: X chromosome, hybrid Testes from for days old individuals (D. simulans, D. sechellia, hybrid D. simulans female x D. sechellia male, hybrid D. sechellia female x D. simulans male) were dissected and RNA was extracted and hybridized along with a reference RNA from the whole body of 4 days old D. melanogaster male. Gene expression in hybrids were compared to parental gene expression in order to isolate misexpressed genes in each hybrids. In order to reveal the cross effect misexpressed genes in hybrids were compared to identify genes commonly misexpressed and genes genes misexpressed in only one hybrid.

性染色体，尤其是X染色体，已被证实对杂种雄性不育具有显著影响。本研究利用拟暗果蝇（D. simulans）与塞氏果蝇（D. sechellia）的正反交杂种，揭示了这类染色体对雄性杂种基因表达的影响。关键词：X染色体，杂种。选取4日龄个体（拟暗果蝇、塞氏果蝇、拟暗果蝇雌性×塞氏果蝇雄性杂种、塞氏果蝇雌性×拟暗果蝇雄性杂种）的睾丸进行解剖，提取RNA，并与来自4日龄黑腹果蝇（D. melanogaster）雄性整体的参考RNA进行杂交实验。为筛选各杂种中的异常表达基因，我们将杂种的基因表达水平与亲本的基因表达水平进行比对。为揭示正反交效应，我们进一步比对不同杂种的异常表达基因，以区分共同异常表达的基因与仅在单一杂种中出现异常表达的基因。